Phylogenetic Analysis of Chlamydia abortus Isolated from fetus Aborted ewes of Alborz Province

Chlamydia is an obligate intercellular and Gram negative coccobacilli and one of the most important factors of abortion in ruminants especially in ewes. Purpose of this investigation Phylogenetic Analysis of Chlamydia abortus Isolated from fetus Aborted ewes of Alborz Province. In this study, of 100 aborted fetuses abomasum of 32 sheeps flocks were Alborz Province, DNA was then extracted IGS-SrRNA using gene specific primers and PCR amplification was performed,and the positive samples were isolated from 10 randomly for sequencing was sent to Macrogene company in Korea.In this study, in total, 37 samples of 100 aborted foetusis were positive for Chlamydia abortus. After sequencing, recording studied gene in the NCBI gene bank operates BLAST, phylogenetic analysis carried out more than 99% of the positive samples with similar gene sequences in database. The sequencing results that isolates clustered together lik 100% and indicated that isolates LN554882/1,AF051935/1 and CR848038/1 was very similar. The isolates studied, we are in a cluster. As well present investigation indicated that Chlamydia abortus is one of the main reasons of ewes abortion in Alborz province.